Structure, Processing and Catalytic Action of Penicillin Acylase
نویسندگان
چکیده
منابع مشابه
Production of Penicillin Acylase.
The production of penicillin acylase by Escherichia coli Ny.I/3-67 has been increased by phenylacetic acid and phenoxyacetic acid, which themselves strongly inhibit the function of this specific enzyme. Other carbonic acids also increased penicillin acylase production, but to a lesser degree; they also weakly inhibited enzyme function. The production of this enzyme was effectively repressed wit...
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Penicillin acylases (PA) are widely used for the production of semi-synthetic β-lactam antibiotics and chiral compounds. In this review, the latest achievements in the production of recombinant enzymes are discussed, as well as the results of PA type G protein engineering.
متن کاملOverexpression of Penicillin V Acylase from Streptomyces lavendulae and 1 Elucidation of its Catalytic Residues
a Department of Biochemistry and Molecular Biology I, Faculty of Biology, University 7 Complutense of Madrid, Madrid, Spain; and b Department of Environmental Biology, Centro 8 de Investigaciones Biológicas, CSIC, Madrid, Spain 9 10 Running head: Approach to catalytic mechanism of Penicillin V acylase from Streptomyces 11 lavendulae 12 #Address correspondence to Isabel de la Mata, [email protected]...
متن کاملOverexpression of penicillin V acylase from Streptomyces lavendulae and elucidation of its catalytic residues.
The pva gene from Streptomyces lavendulae ATCC 13664, encoding a novel penicillin V acylase (SlPVA), has been isolated and characterized. The gene encodes an inactive precursor protein containing a secretion signal peptide that is activated by two internal autoproteolytic cleavages that release a 25-amino-acid linker peptide and two large domains of 18.79 kDa (alpha-subunit) and 60.09 kDA (beta...
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The crystallization of three catalytically inactive mutants of penicillin V acylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoprote...
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ژورنال
عنوان ژورنال: Biotechnology and Genetic Engineering Reviews
سال: 1990
ISSN: 0264-8725,2046-5556
DOI: 10.1080/02648725.1990.10647869